58672C

GS Supplement 50X

50 ×, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): GSEM Supplement, Glutamine synthetase expression medium supplement
• MDL number: MFCD00677096
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• description: for research or for further manufacturing use
• Quality Level: 500
• sterility: sterile-filtered
• form: liquid
• concentration: 50 ×
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The GS System^™ uses the Glutamine synthetase (GS) gene as a selectable marker for the expression of recombinant proteins in mammalian cells, especially NS0 and CHO cells. The system has been shown to have significant advantages with respect to speed and efficiency of cell line development and has resulted in high productivity for antibodies and other recombinant proteins.

Application

GS Supplement 50X has been used to as a supplement for culture media to grow GS-NS0 mouse myeloma cells.[1]

The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H₂O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Storage and Stability

Store GS Supplement 50X protected from light at -10 to -40 °C.

Legal Information

GS System is a trademark of Lonza Group Ltd.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves