The long-term stability of this product in solution has not been determined. However, a similar Collagenase type IV product, C5138, notes that solutions are stable for several months when stored in aliquots at -20°C. Please see the link below to review the datasheet of a similar item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/876/c5138pis.pdf
C4-BIOC
Collagenase Type IV, Cls IV
Synonym(s):
Collagen A, Type IV Gelatinase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352204
NACRES:
NA.78
Pricing and availability is not currently available.
Recommended Products
Looking for similar products? Visit Product Comparison Guide
General description
Collagenase Type IV, Cls IV is a member of the mammalian extracellular neutral metalloproteinases family. It digests type IV collagen, which is a major structural constituent of the basement membrane.[1]
This product is identical to Biochrom GmbH part numbers C4-22 and C4-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities. Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.
Application
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients[2]
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats[3]
- in the isolation of rat glomerular endothelial cells (GEndCs)[4]
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Zongliang Zhang et al.
Journal of translational medicine, 21(1), 23-23 (2023-01-13)
Chimeric antigen receptor (CAR) T cells and immune checkpoint blockades (ICBs) have made remarkable breakthroughs in cancer treatment, but the efficacy is still limited for solid tumors due to tumor antigen heterogeneity and the tumor immune microenvironment. The restrained treatment
Yaru Huangfu et al.
Frontiers in pharmacology, 14, 1105726-1105726 (2023-02-07)
Severe acute pancreatitis (SAP) is a lethal gastrointestinal disorder, yet no specific and effective treatment is available. Its pathogenesis involves inflammatory cascade, oxidative stress, and autophagy dysfunction. Xanthohumol (Xn) displays various medicinal properties, including anti-inflammation, antioxidative, and enhancing autophagic flux.
Weixing Dai et al.
Cancer communications (London, England), 42(9), 848-867 (2022-07-30)
Abnormal expression of protein tyrosine phosphatases (PTPs) has been reported to be a crucial cause of cancer. As a member of PTPs, protein tyrosine phosphatase receptor type O (PTPRO) has been revealed to play tumor suppressive roles in several cancers
Sina M Schalbetter et al.
Science advances, 8(9), eabi6672-eabi6672 (2022-03-03)
The prefrontal cortex (PFC) is a cortical brain region that regulates various cognitive functions. One distinctive feature of the PFC is its protracted adolescent maturation, which is necessary for acquiring mature cognitive abilities in adulthood. Here, we show that microglia
Mingtai Gui et al.
Experimental and therapeutic medicine, 22(6), 1446-1446 (2021-11-02)
Continuous and irreversible cardiac hypertrophy can induce cardiac maladaptation and cardiac remodeling, resulting in increased risk of developing cardiovascular diseases. The present study was conducted to investigate the therapeutic effect of Huoxue Qianyang Qutan recipe (HQQR) on angiotensin II (Ang
-
What is the storage condition and shelf life after reconstituting the product?
1 answer-
Helpful?
-
-
How does one solubilize collagenase, and what is the usage concentration for digestion and cell isolation?
1 answer-
The substance is soluble at 50mg/ml and can be dissolved in water, although it is recommended to use saline, NaCl, DPBS, Hanks, or a culture medium. It is worth noting that in peer-reviewed papers at sial.com, glomerular endothelial cells were isolated at 0.1% in DMEM.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
