11582950001
Roche
BM Chemiluminescence ELISA Substrate (POD)
Synonym(s):
ELISA substrate
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352204
Recommended Products
![BM Chemiluminescence Western Blotting Substrate (POD) pkg of 1 set (11500708001 [1,000 cm2 membrane]), pkg of 1 set (11500694001 [4,000 cm2 membrane])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
form
solution
Quality Level
packaging
pkg of 250 mL (for 2500 wells or 1000 tubes)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
ELISA: suitable
color
clear colorless
solubility
water: miscible
suitability
suitable for chemiluminescence
shipped in
wet ice
storage temp.
2-8°C
General description
The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.
Application
The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.
Features and Benefits
- Improved sensitivity
- Large dynamic range
- Rapid and constant signal
Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)
Principle
Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.
Preparation Note
Working solution: Preparation of working solution
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
- Add to 100 parts of solution A one part of solution B.
- Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Hyeseon Cho et al.
Science translational medicine, 13(616), eabj5413-eabj5413 (2021-09-15)
[Figure: see text].
Suvoshree Ghosh et al.
Journal of thrombosis and haemostasis : JTH, 19(6), 1412-1424 (2021-02-17)
Vitamin K dependent coagulation factor deficiency type 1 (VKCFD1) is a rare hereditary bleeding disorder caused by mutations in γ-glutamyl carboxylase (GGCX). VKCFD1 patients are treated life-long with high doses of vitamin K in order to correct the bleeding phenotype.
Cherrelle Dacon et al.
Cell host & microbe (2022-11-09)
Humanity has faced three recent outbreaks of novel betacoronaviruses, emphasizing the need to develop approaches that broadly target coronaviruses. Here, we identify 55 monoclonal antibodies from COVID-19 convalescent donors that bind diverse betacoronavirus spike proteins. Most antibodies targeted an S2
Extracellular matrix remodeling proteins as biomarkers for clinical assessment and treatment outcomes in eosinophilic esophagitis
Pehrsson M, et al.
BMC Gastroenterology, 23(1) (2023)
F Gagné et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 153(1), 99-106 (2010-09-28)
The endocrine-disrupting activity of municipal effluents has the potential to alter the reproductive system and induce feminization to aquatic organisms. The purpose of this study was to examine the sex ratio, vitellogenin (Vtg)-like proteins, serotonin, arachidonate cyclooxygenase (COX) activity and
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service