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Roche

Nutridoma™-CS

suitable for hybridoma, solution, pkg of 10 mL (50x)

Synonym(s):

serum replacements

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About This Item

UNSPSC Code:
12352207

sterility

sterile; 0.2 μm filtered

Quality Level

100

form

solution

packaging

pkg of 10 mL (50x)

manufacturer/tradename

Roche

concentration

50 ×

technique(s)

cell culture | hybridoma: suitable

impurities

mycoplasma, tested
100 EU/mL endotoxin (LAL test)

solubility

water: miscible

storage temp.

−20°C

General description

Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.

Application

Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.

Unit Definition

Endotoxin (LAL): 100 EU/ml, mycoplasma tested

Physical form

Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.

Preparation Note

Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Nutridoma is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mariana Capurro et al.
Gastroenterology, 125(1), 89-97 (2003-07-10)
Early detection of hepatocellular carcinoma (HCC) is critical for successful treatment. However, the differential diagnosis between HCC and benign hepatic lesions is sometimes difficult and new biochemical markers for HCC are required. It has been reported that glypican-3 (GPC3) messenger
Debora Steiner et al.
Nature biotechnology, 28(4), 361-364 (2010-03-31)
Undifferentiated human embryonic stem cells (hESCs) are currently propagated on a relatively small scale as monolayer colonies. Culture of hESCs as floating aggregates is widely used for induction of differentiation into embryoid bodies. Here we show that hESC lines can
Oliver Sterthaus et al.
Cellular reprogramming, 16(3), 185-195 (2014-05-09)
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four lines. The observed differences were typical of

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