Traditional DNA/RNA isolation kits can be used to prepare genomic material for sequencing (RNA-seq, scRNA-seq, WGS, CHIP-seq etc). For some applications with large organoids, tissue specific DNA/RNA extraction kits are recommended. TRI Reagent (T9424) can be added to the dissociated organoids before RNA extraction and can be stored at -80 °C until future use.
SCC310
3dGRO® Colon Intestinal Organoids, Age 46 (14-881-CR)
Cryopreserved patient derived gastrointestinal organoids (PDOs)
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About This Item
UNSPSC Code:
41106509
NACRES:
NA.81
Pricing and availability is not currently available.
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technique(s)
cell culture | stem cell: suitable
General description
Patient derived organoids (PDOs) are novel in vitro 3D cell models that preserve original tissue physiology and molecular pathology, thus representing a clinically-relevant alternative to traditional 2D cell lines and an effective tool to refine and reduce animal models. PDOs can be derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations and cultured within an ECM-rich substrate using specialized organoid media such as L-WRN conditioned media. Adult tissue derived organoids are phenotypically and genetically stable in long term culture, presenting more mature phenotypes compared to iPSC-derived organoids. Importantly, PDOs have shown to be able to predict patient clinical responses to chemotherapeutics.
We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.
Cell Line Characteristics
We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.
Cell Line Characteristics
- Sex: Male
- Age: 46 Years
- Organ: Colon (Rectum)
- Disease: Adenocarcinoma (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs)
Cell Line Origin
Human, Organoid
Packaging
≥1500 viable organoids/vial
Storage and Stability
Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Legal Information
3dGRO® organoids were derived utilizing HUB Organoid Technology
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. The purchaser of this product shall agree to HUB′s terms of use, which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this Product to purchaser.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
Protocols
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
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Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.
Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.
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How Do you Extract RNA/DNA from Organoids in Cultured in Matrigel?
1 answer-
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How are organoids cultured?
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Once isolated, organoids are cultured in specialized media embedded in a rich extracellular matrix (ECM) basement membrane extract hydrogel such as Growth Factor Reduced Matrigel. Media is replenished every other day and cells are passaged once per week (7-12 days) before cells become too large or necrotic. Cells can be passaged in either small clump fragments or as single cells using mechanical dissociation/enzyme-free passaging reagents. Depending on organoid type and confluency, split ratios of roughly 1:3-1:4 can be used when passaging organoids. For sensitive organoid types, ROCKi (Y27632) (SCM075) can be added during passaging to help in promoting cell viability.
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How do you stain organoids with antibodies?
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Organoids can be stained with antibodies for immunocytochemical (ICC)/Immunohistochemical (IHC) analysis using either whole-mount or paraffin embedding/sectioning techniques. It is critical to use antibodies prequalified for organoid analysis.
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