Skip to Content
Merck
All Photos(3)

Documents

MABE1076

Sigma-Aldrich

Anti-mono- ADP-ribose binding reagent

from Escherichia coli

Synonym(s):

Binding Reagent

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

Escherichia coli

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human, mouse

packaging

antibody small pack of 25 μg

technique(s)

dot blot: suitable
western blot: suitable

shipped in

dry ice

target post-translational modification

unmodified

General description

Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.

Specificity

This reagent binds to mono-ADP ribosylated proteins.

Application

Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.

Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

Quality

Evaluated by Gel Electrophoresis.


Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.

Target description

Variable depending on the target proteins and the extent of ADP-ribosylation.

Physical form

Format: Purified
Immobilized Metal Affinity Chromatography (IMAC)
Purified from E. coli and supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol, with 10% glycerol without preservatives.

Storage and Stability

Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ning-Ning Zhang et al.
Oncology reports, 43(5), 1413-1428 (2020-04-24)
Colorectal cancer (CRC) is a global health concern. The role of epigenetics in tumors has garnered increasing interest. ADP ribosylation is an epigenetic modification that is associated with a variety of biological functions and diseases, and its association with tumor
Yanmei Zhang et al.
Molecular cancer, 21(1), 158-158 (2022-08-03)
Brother of regulator of imprinted sites (BORIS) is expressed in most cancers and often associated with short survival and poor prognosis in patients. BORIS inhibits apoptosis and promotes proliferation of cancer cells. However, its mechanism of action has not been
Alvin Gomez et al.
The Biochemical journal, 475(23), 3827-3846 (2018-10-31)
Here, we report the biochemical characterization of the mono-ADP-ribosyltransferase 2,3,7,8-tetrachlorodibenzo-p-dioxin poly-ADP-ribose polymerase (TIPARP/ARTD14/PARP7), which is known to repress aryl hydrocarbon receptor (AHR)-dependent transcription. We found that the nuclear localization of TIPARP was dependent on a short N-terminal sequence and its
Lisa Weixler et al.
Life science alliance, 6(1) (2022-11-12)
The modification of substrates with ADP-ribose (ADPr) is important in, for example, antiviral immunity and cancer. Recently, several reagents were developed to detect ADP-ribosylation; however, it is unknown whether they recognise ADPr, specific amino acid-ADPr linkages, or ADPr with the
Oney Ortega Granda et al.
Virology, 587, 109845-109845 (2023-07-31)
SARS-CoV-2 is a large, enveloped and positive sense single stranded RNA virus. Its genome codes for 16 non-structural proteins. The largest protein of this complex is nsp3, that contains a well conserved Macro1 domain. Viral Macro domains were shown to

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service