We do not provide a recommended protocol for 384-well conversion. However, the Custom Assay Team (CADI) offers this as a service.
48-680MAG
MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay
Synonym(s):
ERK Cell Signaling Assay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Pricing and availability is not currently available.
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species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
storage temp.
2-8°C
General description
The Milliplex® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70S6K (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
Analyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (Thr180/Tyr182), p70S6K (Thr412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473).
Research Category
Cell Signaling
Cell Signaling
Research Sub Category
Oncology
Oncology
Used to detect/quantify:CREB (pS133)|ERK (pT185/pY187)|NFκB (pS536)|JNK (pT183/pY185)|p38 (Thr180/Tyr182)|p70S6K (Thr412)|STAT3 (pS727)|STAT5A/B (pY694/699)|Akt (pS473)
Storage and Stability
Recommended storage for kit components is 2 - 8°C
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The endocannabinoid anandamide (AEA) causes vasorelaxation in animal studies. Although circulating AEA levels are increased in many pathologies, little is known about its vascular effects in humans. The aim of this work was to characterise the effects of AEA in
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Granulocyte-macrophage colony-stimulating factor auto-antibodies (GMAbs) suppress neutrophil-extrinsic GM-CSF signaling and increase risk for stricturing behavior in Crohn's disease (CD). We aimed to define clinical, genomic, and functional associations with neutrophil-intrinsic GM-CSF signaling. Missense mutations in CSF2RA, CSF2RB, JAK2, STAT5A, and
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Scientific reports, 11(1), 7023-7023 (2021-03-31)
Natural killer (NK) cells mediate innate host defense against microbial infection and cancer. Hypoxia and low glucose are characteristic for these tissue lesions but do not affect early interferon (IFN) γ and CC chemokine release by interleukin 15 (IL-15) primed
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Some human observational studies have suggested an anti-inflammatory role of osteocalcin (OCN). An inflammatory protocol using interferon-γ and tumor necrosis factor-α (10 ng/ml) was employed to examine the acute (24 hr) and chronic (144 hr) effects of uncarboxylated OCN (ucOCN) in commercial, primary
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Is there a protocol available for running the 96-well Milliplex kits in a 384-well format?
1 answer-
Helpful?
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Can you explain what the Amplification Buffer is in Cell Signaling Panels?
1 answer-
The use of the Amplification Buffer is proprietary. Its primary purpose is to enhance the signal of phosphorylated proteins.
Helpful?
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Are the Milliplex Cell Signaling panels qualitative? If so, what settings are used in xPonent for analysis? Can the data be normalized in Belysa?
1 answer-
The Cell Signaling kits are qualitative, and the xPonent Analysis method is not applicable. Belysa does not offer a qualitative option.
Helpful?
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