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860891
Bromocresol Purple sodium salt
indicator grade, Dye content 90 %
Synonym(s):
5′,5″-Dibromo-o-cresolsulfonphthalein sodium salt
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About This Item
Empirical Formula (Hill Notation):
C21H15Br2NaO5S
CAS Number:
Molecular Weight:
562.20
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
grade
indicator grade
Quality Level
form
powder
composition
Dye content, 90%
technique(s)
titration: suitable
color
brown to very dark brown, to Very Dark Red
visual transition interval
5.2-6.8, yellow to purple
mp
255 °C (dec.) (lit.)
λmax
379 nm
585 nm (2nd)
ε (extinction coefficient)
≥47000 at 587-591 nm in 0.1 M NaOH
≥7000 at 375-381 nm in 0.1 M NaOH
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4
InChI
1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1
InChI key
WNOIORUCCYNQPE-UHFFFAOYSA-M
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General description
Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.
Application
Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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