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T8158

Sigma-Aldrich

Transferrin human

powder, BioReagent, suitable for cell culture

Synonym(s):

Siderophilin, partially saturated

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About This Item

CAS Number:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00132442
UNSPSC Code:
12352202
NACRES:
NA.75

product line

BioReagent

Assay

≥98%

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested
 hepatitis and HIV antibody, none detected

solubility

H2O: 50 mg/mL

UniProt accession no.

P02787

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... TF(7018)

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General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:
  • to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
  • as a supplement in serum-containing/devoid medium for human thyroid cell culture
  • as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Martijn F Hoes et al.
European journal of heart failure, 20(5), 910-919 (2018-02-28)
Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine
Cells: Molecules and Mechanisms, 174-174 (2009)
Non-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot P, et al.
Biochim. Biophys. Acta Gen. Subj., 1820(3), 403-410 (2012)
Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels
Yoshimura T, et al.
Analytical Biochemistry, 423(2), 253-260 (2012)
A Dautry-Varsat et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(8), 2258-2262 (1983-04-01)
At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to
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Articles

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

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