This kit has not been tested for use with Porcine plasma. The end user would have to determine suitability. Note that in the publication provided in the link below, the sequence of mature circulating Atrial Natriuretic Peptide (ANP) in human and porcine is identical.
https://pmc.ncbi.nlm.nih.gov/articles/PMC4855512/
RAB0385
Atrial Natriuretic Peptide EIA Kit
for serum, plasma, culture supernatant and cell lysates
Synonym(s):
Natriuretic peptides A, Prepronatriodilatin, ANP
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Pricing and availability is not currently available.
species reactivity
mouse, human, rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, enzyme immunoassay: suitable
input
sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NPPA(4878)
General description
The ANP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting ANP peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANP-1 targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.
Immunogen
Atrial Natriuretic Peptide, synthetic peptide
Application
Atrial Natriuretic Peptide EIA Kit has been used for the quantification of atrial natriuretic peptide (ANP) in cell culture.
For research use only. Not for use in diagnostic procedures.
Atrial Natriuretic Peptide EIA Kit has been used to measure ANP levels in diabetes mellitus patients.
Atrial Natriuretic Peptide EIA Kit has been used to measure ANP levels in diabetes mellitus patients.
Biochem/physiol Actions
Atrial natriuretic peptide (ANP) is a polypeptide hormone released in response to atrial dilation and it stimulates diuresis, natriuresis and vasorelaxation. ANP acts as an inhibitor of renin-angiotensin-aldosterone system (RAAS) pathway. It also has a major role in body fluid homeostasis and maintenance of blood pressure. Binding of ANP to receptors on target cell stimulate the production of cyclic guanosine monophosphate (GMP). Overexpression of ANP leads to congestive heart failure in the human. Experimental studies show that ANP can be used as a therapeutic for stimulating angiogenesis in ischemic tissues. N-terminal ANP prohormone functions as a biomarker for asymptomatic systolic left ventricular (LV) dysfunction, congestive heart failure and cardiovascular mortality.
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| description for serum, plasma, culture supernatant and cell lysates | description for serum, plasma, culture supernatant and cell lysates | description for serum, plasma, culture supernatant and cell lysates | description for serum, plasma, culture supernatant and cell lysates |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) enzyme immunoassay: suitable, ELISA: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) enzyme immunoassay: suitable, ELISA: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable |
| species reactivity mouse, human, rat | species reactivity human, mouse, rat | species reactivity human, mouse, rat | species reactivity human, rat, mouse |
| Gene Information human ... NPPA(4878) | Gene Information human ... NPPB(4879) | Gene Information human ... INS(3630) | Gene Information human ... GHRL(51738) |
| assay range inter-assay cv: <15% | assay range inter-assay cv: <15% | assay range inter-assay cv: <15% | assay range inter-assay cv: <15% |
| input sample type serum | input sample type serum | input sample type plasma | input sample type culture supernatant(s) |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
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can this product be used in porcine plasma?
1 answer-
Helpful?
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Does this ELISA measure N-terminal or C-terminal ANP?
1 answer-
The ANP EIA kit can detect both N- and C- terminal since ANP has only 28 peptides.
Helpful?
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