This product is not assigned an expiration date. When stored at -20 °C, as recommended, the material is stable for at least two years from the date of Quality Release. The recommended retest date is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/ppc1010#product-documentation
Please see the following link to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdf
PPC1010
Protease and Phosphatase Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase
Synonym(s):
Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
Pricing and availability is not currently available.
Product Name
Protease and Phosphatase Inhibitor Cocktail,
form
DMSO solution
Quality Segment
packaging
pkg of 1 ml, 5 ml ×
input
general use sample(s)
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
General description
Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.
Application
Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
- for cellular protein extraction
- to lyse purified CD4+ T cells
- to prepare protein extracts from placental tissues and cells
Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.
Other Notes
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
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How log can you store it at -20 degree? Is it stable?
1 answer-
Helpful?
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Does PPC1010 contain EDTA?
1 answer-
PPC1010 does not contain EDTA
Helpful?
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Does these Protease and Phosphatase Inhibitor Cocktail contains any type of metal chelator or EDTA?
1 answer-
This product does not contains EDTA or other chelators. See the link below to review the product datasheet which includes the cocktail components:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdfHelpful?
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