The isotype of C2206 is Rabbit IgG.
The immunogen corresponds to amino acids 768–781 of human/mouse ?-catenin, supporting cross-reactivity with both species. For additional species, cross-reactivity may occur based on sequence homology; however, it is not guaranteed and depends on the degree of conservation within the ?-catenin sequence. Species-specific reactivity data is not available for all organisms; however, based on sequence homology, this epitope is expected to cross-react with zebrafish, bovine, rat, and potentially several additional species. Please check this UniProt link for details;
https://www.uniprot.org/blast/uniprotkb/ncbiblast-R20260504-162248-0657-52013361-p2m/overview
C2206
Anti-β-Catenin antibody produced in rabbit
whole antiserum
Synonym(s):
Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, ICC, IHC, WB
Citations:
389
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen 88-94 kDa
contains
15 mM sodium azide
species reactivity
several mammalian species
packaging
antibody small pack of 25 μL
technique(s)
dot blot: suitable using the immunogen and related peptides conjugated to BSA, immunocytochemistry: 1:2,000 using cultured MDBK cells, immunohistochemistry: 1:2,000 using bovine kidney frozen sections, western blot: 1:4,000 using cultured MDBK cells
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
General description
Catenin are distinct peripheral cytosolic proteins and are classified into α-, β- and γ-catenin (102 kDa, 94 kDa and 86 kDa respectively. It is found in varying abundance in many developing and adult tissues.
Immunogen
synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.
Application
Anti-β-Catenin antibody produced in rabbit has been used in
- co-immunoprecipitation
- immunoblotting
- immunofluorescence
- immunoperoxidase assay
Biochem/physiol Actions
Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The protein, β-catenin, binds directly to the cytoplasmic tail of E-cadherin. Plakoglobin (probably identical to γ-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene Adenomatous polyposis coli (APC). The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and wingless-related integration site (Wnt-1). p120cas which exhibits structural similarity to β-catenin and plakoglobin may represent another catenin associated with cadherin.
The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| antibody form whole antiserum | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| technique(s) dot blot: suitable using the immunogen and related peptides conjugated to BSA, immunohistochemistry: 1:2,000 using bovine kidney frozen sections, immunocytochemistry: 1:2,000 using cultured MDBK cells, western blot: 1:4,000 using cultured MDBK cells | technique(s) immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract, immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells, immunohistochemistry: suitable, immunoprecipitation (IP): suitable | technique(s) indirect ELISA: suitable, microarray: suitable, western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132 | technique(s) immunocytochemistry: 1:50 using 293 T (human embryonic kidney cell), immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 8-10 μg/mL using extract of cultured human 293T cells treated with Lici (20 mM) and calyculin A (50 nM) |
| clone polyclonal | clone 15B8, monoclonal | clone BC-22, monoclonal | clone BCT-41, monoclonal |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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what is the isotype and the reactivity (which are these "several mammalian species" )?
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