51280

Hemin

BioXtra, from Porcine, ≥96.0% (HPLC)

• Synonym(s): Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)
• Empirical Formula (Hill Notation): C₃₄H₃₂ClFeN₄O₄
• CAS Number: 16009-13-5
• Molecular Weight: 651.94
• Beilstein: 5229914
• EC Number: 240-140-1
• MDL number: MFCD00010726
• UNSPSC Code: 12352202
• PubChem Substance ID: 57651319
• NACRES: NA.79

Properties

• biological source: Porcine
• product line: BioXtra
• Assay: ≥96.0% (HPLC)
• form: powder or crystals
• technique(s): tissue culture: suitable; transfection: suitable
• storage temp.: 2-8°C
• SMILES string: Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C
• InChI: 1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;
• InChI key: BTIJJDXEELBZFS-HXFTUNQESA-K

Description

Application

Hemin is used for the following applications:

• To study bacterial strains and growth conditions
• Used in short-term colony assays (the culture medium consists of hemin along with other components)
• Cell transfections

It may be used in β-hematin formation assay for analyzing the inhibitory activity of MMV (Medicines for Malaria Venture) Malaria Box compounds against the formation of β-hematin.

Biochem/physiol Actions

Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.

Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves