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04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonym(s):

Staining kit for live/dead cells

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

suitability

suitable for fluorescence

Quality Level

100

storage temp.

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Kit Components Only

Product No.
Description
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Certificates of Analysis (COA)

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T Kimura et al.
Neuroscience letters, 208(1), 53-56 (1996-04-12)
The recent immunological demonstration of advanced glycation end products (AGE) of the Maillard reaction in several human tissues suggests a possible involvement of AGE in the aging process. We previously prepared a monoclonal anti-AGE antibody (6D12) which recognized N epsilon-(carboxymethyl)lysine.
T Matsuse et al.
Journal of clinical pathology, 51(7), 515-519 (1998-11-03)
To investigate the presence and distribution of advanced glycation end products (AGE) in pulmonary fibrosis. Lung tissue samples obtained from seven necropsy cases with idiopathic pulmonary fibrosis and seven with normal pulmonary parenchyma were examined immunohistochemically with a monoclonal antibody
T Meshulam et al.
The Journal of infectious diseases, 172(4), 1153-1156 (1995-10-01)
Studies of antimycotic host defenses have been limited by the paucity of rapid, reproducible quantitative assays for fungal cell damage. Prior studies defined a colorimetric method that uses MTT, a tetrazolium dye, to quantify polymorphonuclear leukocyte (PMNL)-mediated damage to fungi.
Amin Hassanzadeh-Barforoushi et al.
Lab on a chip, 18(15), 2156-2166 (2018-06-21)
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized
Camille Raillon et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(10), 1085-1095 (2019-08-01)
The isolation, analysis, and enumeration of circulating tumor cells (CTCs) from cancer patient blood samples are a paradigm shift for cancer patient diagnosis, prognosis, and treatment monitoring. Most methods used to isolate and enumerate these target cells rely on the
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