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Key Documents

90903

Sigma-Aldrich

Schaeffer and Fulton Spore Stain Solution A

for microscopy

Synonym(s):

Malachite Green Solution according to Schaeffer and Fulton

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About This Item

CAS Number:
2437-29-8
MDL number:
MFCD06412418
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

product line

BioChemika

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

spore forming microorganisms

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

InChI key

CNYGFPPAGUCRIC-UHFFFAOYSA-L

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Components

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Statements

H318,H361fd,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK0823
BCCK9351
BCCJ6790
BCCH6741
BCCG1482

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