MABS195
Anti-Pan-Ras Antibody, clone RAS 10
clone RAS 10, from mouse
Synonym(s):
GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase Kras, N-terminally processed
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RAS 10, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRAS(3845)
General description
The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
Immunogen
Recombinant protein corresponding to human Pan-Ras.
Application
Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Quality
Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Target description
~21 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
MCF7 cell lysate
MCF7 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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F Roßner et al.
Oncogenesis, 5, e187-e187 (2016-01-19)
The transcription factor YBX1 can act as a mediator of signals transmitted via the EGFR-RAS-MAPK axis. YBX1 expression has been associated with tumor progression and prognosis in multiple types of cancer. Immunohistochemical studies have revealed dependency between YBX1 expression and
Michele Caraglia et al.
International journal of cancer, 121(10), 2317-2330 (2007-07-28)
Interferon alpha (IFNalpha) induces an EGF-Ras-->Raf-1-->Erk dependent survival pathway counteracting apoptosis induced by the cytokine. In this paper we have evaluated the effects of the combination between farnesyl-transferase inhibitor (FTI) R115777 and IFNalpha on the growth inhibition and apoptosis of
Won-Ji Ryu et al.
Experimental & molecular medicine, 52(5), 832-842 (2020-05-28)
Triple-negative breast cancer (TNBC) is a severe and heterogeneous disease that lacks an approved targeted therapy and has a poor clinical outcome to chemotherapy. Although the RAS-ERK signaling axis is rarely mutated in TNBC, ~50% of TNBCs show an increased
Taras A Redchuk et al.
Nature communications, 11(1), 605-605 (2020-02-01)
Techniques of protein regulation, such as conditional gene expression, RNA interference, knock-in and knock-out, lack sufficient spatiotemporal accuracy, while optogenetic tools suffer from non-physiological response due to overexpression artifacts. Here we present a near-infrared light-activatable optogenetic system, which combines the
Charlotte A Townsend et al.
STAR protocols, 5(2), 103068-103068 (2024-05-19)
S-acylation, commonly palmitoylation, is the addition of fatty acids to cysteines to regulate protein localization and function. S-acylation detection has been hampered by limited sensitivity and selectivity in low-protein, costly samples like cultured neurons. Here, we present a protocol for
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