MABF509

Anti-CD115 (c-fms) Antibody (mouse), PE, clone AFS98

clone AFS98, 0.2 mg/mL, from rat

• Synonym(s): Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1-R
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: AFS98, monoclonal
• species reactivity: mouse
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG2aκ
• UniProt accession no.: P09581
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Csf1R(12978)

Description

General description

The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor.

This rat monoclonal antibody Anti-CD115 (c-fms) (mouse), PE, clone AFS98 validated for use in western blotting & Flow Cytometry for the detection of CD115 (c-fms).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD115 (c-fms) in one million C57Bl/6 peripheral blood cells.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable