T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

• Synonym(s): 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
• Empirical Formula (Hill Notation): C₁₆H₂₀N₂ · 2HCl
• CAS Number: 64285-73-0
• Molecular Weight: 313.27
• EC Number: 264-769-6
• MDL number: MFCD00012961
• UNSPSC Code: 12352204
• PubChem Substance ID: 24900140
• NACRES: NA.83

Properties

• Product Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
• form: tablet
• mp: ≥300 °C
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
• InChI key: NYNRGZULARUZCC-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

• anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
• anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
• in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves