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SRE0082

Sigma-Aldrich

Peroxidase from horseradish

Synonym(s):

Peroxidase from horseradish, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

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About This Item

CAS Number:
9003-99-0
Enzyme Commission number:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00071339
UNSPSC Code:
12352204
NACRES:
NA.54

description

For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.

Quality Level

400

specific activity

≥250 units/mg solid (using pyrogallol)

shipped in

wet ice

storage temp.

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application

Peroxidase from horseradish has been used in western blotting.

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificates of Analysis (COA)

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Yonghao Sun et al.
Experimental and therapeutic medicine, 14(2), 1809-1817 (2017-08-16)
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Articles

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

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