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SAE0013

Sigma-Aldrich

Oxyrase ® for Broth

Synonym(s):

OB

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

shipped in

dry ice

storage temp.

−20°C

General description

Oxyrase for Broth is a sterile enzyme formulation that uses the Oxyrase Enzyme System to produce anaerobic conditions in different bacteriological broth media. Oxyrase for Broth facilitates growth of anaerobes, such as for reviving lyophiles. Oxyrase for Broth is a ready-to-use product that renders broth anaerobic in 10-15 minutes, and continues to remove oxygen from the container headspace beyond that time.

Application

Oxyrase® for broth has been used in reinforced clostridial media to provide an anaerobic environment for culturing Bifidobacterium adolescentis. It has also been used as a component in PMM/imaging media for oxygen-free microscopy experiments to remove oxygen.

Biochem/physiol Actions

Oxyrase® for broth is useful to cultivate species like Shigella sonnei and Shewanella oneidensis.It also provides anaerobic conditions for cultivating species like Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.

Storage and Stability

Store at -20 °C. (Long Term Storage)

Analysis Note

Oxyrase contains a penicillin binding protein that may interfere with penicillin and some related antibiotics.

Legal Information

Oxyrase is a registered trademark of Oxyrase, Inc.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Andrew P Jacobson et al.
Journal of AOAC International, 87(5), 1115-1122 (2004-10-21)
Alternative methods of reducing oxygen during anaerobic enrichment in the Bacteriological Analytical Manual (BAM) Shigella culture method were evaluated and compared to the current and less practical GasPak method. The alternative anaerobic methods included the use of reducing agents in
Ken H Liu et al.
Nature metabolism, 3(12), 1694-1705 (2021-12-22)
Obesity and obesity-related metabolic disorders are linked to the intestinal microbiome. However, the causality of changes in the microbiome-host interaction affecting energy metabolism remains controversial. Here, we show the microbiome-derived metabolite δ-valerobetaine (VB) is a diet-dependent obesogen that is increased
Patricia A Bradford et al.
Antimicrobial agents and chemotherapy, 49(9), 3903-3909 (2005-08-30)
Tigecycline is a broad-spectrum glycylcycline antibiotic with activity against not only susceptible gram-positive and gram-negative pathogens but also strains that are resistant to many other antibiotics. In the process of determining quality control (QC) limits for the American Type Culture
Christopher M Brennan et al.
BMC microbiology, 13, 33-33 (2013-02-12)
Hfq is an RNA chaperone protein that has been broadly implicated in sRNA function in bacteria. Here we describe the construction and characterization of a null allele of the gene that encodes the RNA chaperone Hfq in Shewanella oneidensis strain
Joseph L Roberts et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 132, 110831-110831 (2020-10-07)
The gut microbiota is an important contributor to both health and disease. While previous studies have reported on the beneficial influences of the gut microbiota and probiotic supplementation on bone health, their role in recovery from skeletal injury and resultant

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