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Key Documents

I4640

Sigma-Aldrich

Human IgG4 Kappa, Recombinant Protein

purified immunoglobulin, buffered aqueous solution

Synonym(s):

Human IgG4 Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

human

Quality Level

300

conjugate

unconjugated

antibody form

purified immunoglobulin

Assay

>95% (SDS-PAGE)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

General description

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta and is vital for immune defense of the neonate against infections. IgG4, κ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.

Application

The purified IgG4, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunoblotting, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000397586
0000400972
0000400972
0000397586
SLCP9340

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Rafael D Melani et al.
Journal of proteome research, 21(1), 274-288 (2021-12-09)
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy

Questions

1–2 of 2 Questions  

  1. What is the molecular weight of the Human IgG4 Kappa product I4640?

    1 answer

    1. The typical molecular weight for these IGG kappas is 22.5 kDa.

      Helpful?

  2. Could you please provide me with the formulation information for 'I4640'? We have ordered this product for animal injection, and the formulation information will be necessary when it is diluted with injection buffer.

    1 answer

    1. I4640 is recombinant, purified Human IgG4 Kappa provided in a solution of 0.01 M phosphate-buffered saline, pH 7.4.

      Helpful?

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