Skip to Content
Merck
All Photos(3)

Documents

G1890

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

product name

Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

biological source

Porcine skin

Quality Level

sterility

sterile

type

Type A

product line

BioReagent

form

powder

mol wt

50—100 kDa

packaging

pkg of 1 kg
pkg of 100 g
pkg of 500 g

concentration

70—90% Biuret

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

surface coverage

100‑200 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear to hazy, faintly yellow

shipped in

ambient

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

General description

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.

Application

Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Features and Benefits

  • Sterile gelatin sourced from porcine skin.
  • Free from proteases.
  • In the form of gelatin powder for convenient and easy handling.
  • Versatile for use in cell culture and electrophoresis techniques.
  • Provides adequate surface coverage for various applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Graziano Martello et al.
The EMBO journal, 32(19), 2561-2574 (2013-08-15)
Self-renewal of pluripotent mouse embryonic stem (ES) cells is sustained by the cytokine leukaemia inhibitory factor (LIF) acting through the transcription factor Stat3. Several targets of Stat3 have previously been identified, most notably the reprogramming factor Klf4. However, such factors
In vitro derivation of chondrogenic cells from human embryonic stem cells
Human Embryonic Stem Cell Protocols, 77(1), 317-331 (2009)
Małgorzata Kozikowska et al.
Scientific reports, 9(1), 11969-11969 (2019-08-21)
The retention of the embryo in the transfer catheter after embryo transfer (ET) during in vitro fertilization is a very common phenomenon, encountered by even the most experienced operators, and embryos retained in the transfer catheter or its sleeve require
The effects of GDF-5 and uniaxial strain on mesenchymal stem cells in 3-D culture
Farng E, et al.
Clinical Orthopaedics and Related Research, 466(8), 1930-1937 (2008)
Thermosensitive chitosan-gelatin-glycerol phosphate hydrogel as a controlled release system of ferulic acid for nucleus pulposus regeneration
Cheng YH, et al.
Biomaterials, 32(29), 6953-6961 (2011)

Articles

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

See All

Protocols

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service