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Key Documents

F7634

Sigma-Aldrich

Anti-Sheep IgG (whole molecule)–FITC antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Donkey Anti-Sheep IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

donkey

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect immunofluorescence: 1:40

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Sheep IgG (whole molecule)-FITC antibody is specific for sheep IgG subclasses. Donkey anti-sheep IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC).

Specificity

Binds all sheep Igs

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-FITC antibody may be used for immunofluorescence of mouse spleen cells at a working antibody dilution of 1:40. The antibody was used for direct immunofluorescence of rat and human spermatozoa and in FISH analysis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Galina V Mukamolova et al.
Molecular microbiology, 46(3), 623-635 (2002-11-02)
Mycobacterium tuberculosis and its close relative, Mycobacterium bovis (BCG) contain five genes whose predicted products resemble Rpf from Micrococcus luteus. Rpf is a secreted growth factor, active at picomolar concentrations, which is required for the growth of vegetative cells in
L O Goodwin et al.
Molecular human reproduction, 3(3), 255-268 (1997-03-01)
Therapeutic administration of calcium channel-blocking medications has been correlated with reduced mannose receptor expression and iatrogenic human male infertility. In this report, we investigate whether the pharmacological activity of dihydropyridines, which block calcium influx through voltage-dependent calcium channels, contributes to
Yvette J Williams et al.
The British journal of nutrition, 99(1), 100-109 (2007-08-19)
Two rumen protozoa vaccine formulations containing either whole fixed Entodinium or mixed rumen protozoa cells were tested on Merino sheep with the aim of decreasing the number and/or activity of protozoa in the rumen. Negative control (no antigen) and positive
M L Bermingham et al.
Journal of fish diseases, 27(10), 555-571 (2004-10-16)
A 2-year study was carried out on amoebic gill disease (AGD) involving monthly samples of 1+ Atlantic salmon, Salmo salar L., smolts, histological assessment of the gills and analysis of environmental data. Gill pathology was seen before amoebae could be
K Bakou et al.
Mutagenesis, 17(3), 233-239 (2002-04-25)
Fluorescence in situ hybridization (FISH) was used to evaluate spontaneous and aneuploidogen-induced micronucleus frequencies and non-disjunction of chromosomes X and 8 in cultured binucleated lymphocytes of women of two age groups. Demecolcine and vincristine were used as model aneuploidogens to

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