B0287
Monoclonal Anti-FITC−Biotin antibody produced in mouse
clone FL-D6, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-FITC
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FL-D6, monoclonal
form
buffered aqueous solution
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).
Immunogen
FITC-BSA conjugate
Application
Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
- for amplification of signal in immunofluorescence assays
- in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
- enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Kazuyuki Kasahara et al.
Cell host & microbe, 31(6), 1038-1053 (2023-06-07)
The microbes and microbial pathways that influence host inflammatory disease progression remain largely undefined. Here, we show that variation in atherosclerosis burden is partially driven by gut microbiota and is associated with circulating levels of uric acid (UA) in mice
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Peter Stachon et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(10), 2237-2245 (2014-08-12)
Nucleotides such as ATP, ADP, UTP, and UDP serve as proinflammatory danger signals via purinergic receptors on their release to the extracellular space by activated or dying cells. UDP binds to the purinergic receptor Y6 (P2Y6) and propagates vascular inflammation
Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.
Liu, et al.
Cardiovascular Research, 119, 1046-1061 (2023)
Flow-based microimmunoassay
Hayes MA, et al.
Analytical Chemistry, 73(24), 5896-5902 (2001)
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