Skip to Content
Merck
All Photos(1)

Documents

A7058

Sigma-Aldrich

Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

storage temp.

2-8°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting. Working dilution 1:20



Also suitable for dot blot assays and ELISA

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service