A3311

Bovine Serum Albumin

powder, BioXtra

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• biological source: bovine
• Quality Level: 200
• product line: BioXtra
• Assay: ≥98% (agarose gel electrophoresis)
• form: powder
• mol wt: ~66 kDa
• packaging: poly bottle of
• origin: USA origin
• technique(s): cell culture | embryo: suitable
• loss: ≤5%
• pH: 6.5-7.5
• solubility: water: soluble (40 mg/ml)
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Select the right albumin for your application with the Albumin Selection Guide.

This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves