A solid-phase immunoassay procedure for the determination of progesterone in human plasma is described, which utilizes chemiluminescence as the end-point. A progesterone-isoluminol conjugate serves as the chemiluminescent marker. An IgG fraction of antiserum to progesterone-11 alpha-hemisuccinate bovine serum albumin is
An electrochemiluminescence (ECL) biosensor for simultaneous detection of adenosine and thrombin in one sample based on bifunctional aptamer and N-(aminobutyl)-N-(ethylisoluminol) functionalized gold nanoparticles (ABEI-AuNPs) was developed. A streptavidin coated gold nanoparticles modified electrode was utilized to immobilize biotinylated bifunctional aptamer
Journal of chromatography. A, 1170(1-2), 118-121 (2007-10-02)
A simple and convenient end-column chemiluminescence (CL) detection coupled to pressurized capillary electrochromatography (pCEC) was described. Luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) were adopted as mode compounds to evaluate the feasibility of end-column reactor. Detailed analysis of ABEI revealed that the high
Chemical communications (Cambridge, England), 47(17), 4959-4961 (2011-03-25)
A novel strategy for the synthesis of CF-AuNMs by reducing HAuCl(4) with ABEI in aqueous solution at room temperature is reported. No additional stabilizing and reducing reagents are needed, and various morphologies of CF-AuNMs can be obtained. It could be
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a
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