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925B

Sigma-Aldrich

Peroxide Block

3% Hydrogen Peroxide

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24
Pricing and availability is not currently available.

Quality Level

500

description

[For In Vitro Diagnostic Use in Select Regions]

form

aqueous solution

packaging

pkg of 1 L (925B-09)
pkg of 100 mL ready to use (925B-04)
pkg of 15 mL ready to use (925B-02)
pkg of 200 mL ready to use (925B-05)
pkg of 50 mL ready to use (925B-03)

manufacturer/tradename

Cell Marque®

technique(s)

immunohistochemistry: suitable

shipped in

ambient

General description

Peroxide Block is used to block the endogenous peroxide enzymes that naturally occur in tissue sections. This is a necessary step when performing immunohistochemistry and utilizing a horseradish peroxidase enzyme (HRP) label. If this step is eliminated from the protocol, endogenous peroxide enzymes may cause the chromogen to precipitate, thereby causing background staining to occur. Use of this reagent virtually eliminates this possibility.

Physical form

Solution containing hydrogen peroxide

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000350439
0000350442
0000350444
0000350443
0000350443

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Questions

1–3 of 3 Questions  

  1. There is a requirement for large volumes of H2O2 (containers that exceed 1000 mls), please suggest

    1 answer

    1. Peroxide Block is a 3% hydrogen peroxide solution commonly used for performing IHC.

      Helpful?

  2. Can peroxide block as a diluent or how is it used?

    1 answer

    1. The peroxide block is used to neutralize the endogenous peroxidase that arises from using an HRP or Horse Radish Peroxidase kit. It is part of the protocol and is typically used for 10 minutes as one of the steps.

      Helpful?

  3. What is the % of peroxide in peroxide block 925B-09?

    1 answer

    1. a 3% peroxide block solution should be used.

      Helpful?

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