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MAB2223

Sigma-Aldrich

Anti-DNP Antibody, clone 9H8.1

clone 9H8.1, from mouse

Synonym(s):

Dinitrophenol

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

9H8.1, monoclonal

species reactivity (predicted by homology)

all

technique(s)

western blot: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

General description

Dinitrophenol (DNP) is a small water-soluble, hydrophobic ligand that binds to transthyretin at acidic pH and also reduces the formation of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to acclimated bacteria. DNP has been associated with possible therapies for neurodegenerative and neurological disorders.

Specificity

Demonstrated to react with a DNP moiety in a wider range of species. This antibody′s reactivity is expected to be species independent.

Immunogen

KLH-conjugated DNP

Application

This Anti-DNP Antibody, clone 9H8.1 is validated for use in WB for the detection of DNP.

Quality

Evaluated by Western Blot in Oxyblot protein standards.

Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.

Target description

Various kDa observed. The Oxyblot protein standards (S7151) were used to test this antibody. These standards are phosphorylase B, BSA, OVA, Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues per protein.

Physical form

Format: Purified

Analysis Note

Control
Oxyblot protein standards

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rita Araújo et al.
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The Journal of experimental biology, 226(15) (2023-07-20)
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Sessile benthic organisms like oysters inhabit the intertidal zone, subject to alternating hypoxia and reoxygenation (H/R) episodes during tidal movements, impacting respiratory chain activities and metabolome compositions. We investigated the effects of constant severe hypoxia (90 min at ~ 0% O2 ) followed

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