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AB1536

Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody, CT

Chemicon®, from rabbit

Synonym(s):

DBH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rabbit, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000787.3

UniProt accession no.

P09172

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DBH(1621)

Specificity

Dopamine Beta Hydroxylase (DbetaH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.

Immunogen

Epitope: C-terminus
Synthetic peptide, CPTSQGRSPAGPTVVSI-amide, cloned from human (DbH) cDNA (Nagatsu et al. 1990).

Application

Anti-Dopamine β Hydroxylase Antibody, C-terminus is an antibody against Dopamine β Hydroxylase for use in IH & WB.
Immunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed moderate staining on both human and monkey.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junchao Tong et al.
Journal of neurochemistry, 102(5), 1691-1702 (2007-05-09)
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