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Sigma-Aldrich

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, FITC conjugate

Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

phosphorylation (pSer/pThr)

General description

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.

Anti-phospho-Ser/Thr-Pro, MPM-2 (05-368) conjugated to fluorescein-5-EX, succinimidyl ester (FSE)

Application

Detect phospho-Ser/Thr-Pro also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, FITC conjugate (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
For applications in which an FITC conjugate is advantageous, such as ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated in immunocytochemistry on A431 mitotic cells fixed with 3.7% paraformaldehyde

Physical form

TBS, pH 8.0, 0.05% sodium azide and 30% glycerol

Storage and Stability

Stable for 1 year at -20°C from date of shipment. Store in the dark. For maximum recovery of product, centrifuge the vial.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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MYC proteins are major drivers of cancer yet are considered undruggable because their DNA binding domains are composed of two extended alpha helices with no apparent surfaces for small-molecule binding. Proteolytic degradation of MYCN protein is regulated in part by
cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.
Kuang, J, et al.
Molecular Biology of the Cell, 5, 135-145 (1994)
Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.
Yaffe, M B, et al.
Science (New York, N.Y.), 278, 1957-1960 (1997)
Ekaterina P Lamber et al.
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BRCA1, the breast cancer- and ovarian cancer-specific tumor suppressor, can be a transcriptional repressor or a transcriptional activator, depending on the promoter context. To identify the genes activated or repressed by BRCA1, we have analyzed microarray results from cells depleted

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