ZRB1169
Anti-alpha Actinin 2 Antibody, clone 1E11, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-CMD1AA, Anti-CMH23, Anti-MPD6, Anti-MYOCOZ
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1E11, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 103.85 kDa
observed mol wt ~103 kDa
species reactivity
human, mouse
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ACTN2(88)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 1E11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Alpha-Actinin-2. It targets an epitope within 17 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to the first 17 amino acids from the N-terminal region of Alpha-actinin-2 from human skeletal muscle.
Application
Anti-alpha Actinin 2, clone 1E11 ZooMAb , Cat. No. ZRB1169, is a highly specific rabbit recombinant monoclonal antibody that targets alpha-Actinin-2 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha-Actinin-2 in mouse skeletal muscle tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected alpha- Actinin-2 in human skeletal muscle and mouse skeletal muscle tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle cells (SkMC).
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle frozen tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected alpha- Actinin-2 in human skeletal muscle and mouse skeletal muscle tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle cells (SkMC).
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle frozen tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Target description
Alpha-actinin-2 (UniProt: P35609; also known as Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein) is encoded by the ACTN2 gene (Gene ID: 88) in human. Alpha-actinin-2 is expressed in both skeletal and cardiac muscle. It is considered as a bundling protein that acts to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. It has a rod-shaped structure and it homodimerizes in an anti-parallel fashion. Each monomer contains an N-terminal actin-binding region composed of two calponin homology domains (aa 38-142 and 151-257), two C-terminal EF hand domains (aa 753-788 and 789-824), and four tandem spectrin-like repeats (aa 281-391, 401-506, 516-627, and 637-740). It has also been shown to localize to dendritic spines, enriched within the post-synaptic density (PSD). In rat hippocampal neurons loss of alpha-actinin-2 is shown to create an increased density of immature, filopodia-like protrusions that fail to mature into a mushroom-shaped spine during development and its knockdown prevents the recruitment and stabilization of PSD in the spine, resulting in failure of synapse formation. Mutations in ACTN2 gene is known to cause cardiomyopathy with ventricular hypertrophy leading to dyspnea, syncope, collapse, palpitations, and chest pain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hodges, JL, et al. (2014). PLoS ONE 9(7): e101770).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jingxuan Guo et al.
iScience, 27(6), 109954-109954 (2024-06-03)
Hypertension is a major cause of morbidity and mortality in patients with hypertrophic cardiomyopathy (HCM), suggesting a potential role for mechanics in HCM pathogenesis. Here, we developed an in vitro physiological model to investigate how mechanics acts together with HCM-linked myosin
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