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SRP3050

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

CSF-2, MGI-1GM, pluripoietin-a

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 ng/mL

mol wt

14.6 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CSF2(1437)

General description

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Application

Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells.

Biochem/physiol Actions

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells.

Sequence

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 3.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Siddappa N Byrareddy et al.
PloS one, 10(10), e0140689-e0140689 (2015-10-16)
Lectin-like molecules and their receptors are cell surface molecules that have been shown to play a role in either facilitating infection or serving as transporters of HIV/SIV in vivo. The role of these lectin-like molecules in the pathogenesis of HIV/SIV
Candida parapsilosis produces prostaglandins from exogenous arachidonic acid and OLE2 is not required for their synthesis.
Grozer Z, et al.
Virulence, 6, 85-92 (2015)
Association of genetic variations in the CSF2 and CSF3 genes with lung function in smoking-induced COPD.
He JQ
The European Respiratory Journal, 32, 25-34 (2008)
Growth of clonogenic myeloblastic leukemic cells in the presence of human recombinant erythropoietin in addition to various human recombinant hematopoietic growth factors.
Asano Y
Blood, 72, 1682-1686 (1988)
J Nemunaitis et al.
Blood, 77(9), 2065-2071 (1991-05-01)
Forty-seven patients with hematologic neoplasia received recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) by daily 2-hour infusion following allogeneic bone marrow transplantation from HLA-identical sibling donors in a phase I-II dose-escalation trial. Dose levels ranged from 30 to 500 micrograms/m2/d. At

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