Skip to Content
Merck
All Photos(2)

Key Documents

SAB5700592

Sigma-Aldrich

Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein with His tag

Synonym(s):

2019-nCoV S glycoprotein, 2019-nCoV coronavirus spike Protein, 2019-nCoV ncov spike Protein, COVID-19 S protein, Spike glycoprotein, cov spike Protein

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.26

recombinant

expressed in HEK 293 cells

Quality Level

Assay

>97% (SDS-PAGE)

form

liquid

mol wt

133.8 kDa

impurities

<0.1 EU/μg protein (LAL test)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

SARS virus ... S(43740568)

General description

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant SARS-COV-2 S1+S2 ECD (S-ECD) Protein with His tag (Wild type, pre-fusion state plus post-fusion state) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of SARS-COV-2(2019-nCoV) S1+S2 ECD (S-ECD) (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

Biochem/physiol Actions

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.

Storage and Stability

This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service