P8783

Progesterone

powder, BioReagent, suitable for cell culture

• Synonym(s): 4-Pregnene-3,20-dione
• Empirical Formula (Hill Notation): C₂₁H₃₀O₂
• CAS Number: 57-83-0
• Molecular Weight: 314.46
• Beilstein: 1915950
• EC Number: 200-350-6
• MDL number: MFCD00003658
• UNSPSC Code: 12352209
• PubChem Substance ID: 24898978
• NACRES: NA.77
• biological source: synthetic (organic)
• form: powder
• Assay: ≥99%

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• description: synthesized from materials derived from plant source
• product line: BioReagent
• Assay: ≥99%
• form: powder
• potency: 0.1-20 ng/mL
• technique(s): cell culture | mammalian: suitable
• mp: 128-132 °C (lit.)
• solubility: absolute ethanol: 1 mg/mL
• storage temp.: room temp
• SMILES string: CC(=O)[C@H]1CC[C@H]2[C@@H]3CCC4=CC(=O)CC[C@]4(C)[C@H]3CC[C@]12C
• InChI: 1S/C21H30O2/c1-13(22)17-6-7-18-16-5-4-14-12-15(23)8-10-20(14,2)19(16)9-11-21(17,18)3/h12,16-19H,4-11H2,1-3H3/t16-,17+,18-,19-,20-,21+/m0/s1
• InChI key: RJKFOVLPORLFTN-LEKSSAKUSA-N
• Gene Information: human ... ABCB1(5243) , AR(367) , CYP2C9(1559) , EBP(10682) , ESR1(2099) , ESR2(2100) , NR3C1(2908) , NR3C2(4306) , PGR(5241) , SERPINA6(866) ; mouse ... Esr1(13982) ; rat ... Ar(24208) , Nr3c1(24413) , Pgr(25154)

Description

General description

Progesterone is a steroid hormone secreted by the ovaries.[1]

Application

Progesterone has been used:

• as a growth-promoting hormone in basal medium for bovine mammary epithelial cell culture[2]
• to test its neuroprotective abilities in a well-characterized lateral cortical contusion injury (CCI) model[3]
• as an endocytosis inhibitor to study its effect on Coxsackievirus A9 infection on A549 cells[4]

Use in cell culture applications to study sterol regulation of cell signaling and gene expression.

Biochem/physiol Actions

Induces maturation and secretory activity of the uterine endothelium; suppresses ovulation.

Induces maturation and secretory activity of the uterine endothelium; suppresses ovulation. Progesterone is implicated in the etiology of breast cancer.

Progesterone is a hormone that maintains pregnancy. It is essential for embryo formation, development and survival in almost all mammals. This hormone exhibits its action via progesterone receptor, which is specifically expressed in the endometrial stroma throughout pregnancy.[5] It is known to prevent preterm delivery, when administered intramuscularly.[6] Progesterone also controls gonadotropin secretion, activates sexual behavior and triggers ovarian events leading to ovulation. It stimulates the growth and differentiation of the uterus and prevents uterine contractility.[7]

Other Notes

Steroid hormone produced by the corpus luteum.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 20μg/ml stock solution, add 1ml absolute ethanol per mg progesterone, gently swirl to dissolve, add 49ml sterile medium per ml ethanol.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351,H360FD,H362
• Precautionary Statements: P202 - P260 - P263 - P264 - P280 - P308 + P313
• Hazard Classifications: Carc. 2 - Lact. - Repr. 1A
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves