This kit detects reduced thiols such as reduced Glutathione (GSH) and Cysteine. In addition, the product may also be used to detect SH groups in the protein, provided that a standard with known SH groups is used, such as BSA (0.5-1 GH/molecule). The possible interference of Meleimide has not been investigated.
MAK151
Fluorometric Thiol Assay Kit
sufficient for 200 fluorometric tests
Synonym(s):
Thiol Fluorescent Assay Kit
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About This Item
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usage
sufficient for 200 fluorometric tests
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.
Application
Fluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.[1]
Suitability
This kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.
Principle
The Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 μL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Can this kit be used to quantify cysteines from proteins? If so, do I need to reduce disulfide bonds first? Also, does maleimide interfere with the assay?
1 answer-
Helpful?
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Would MAK151 detect reduced thiols like GSH, Cysteine, and NAC, or does it detect total thiol? Additionally, does it detect total protein-SH or protein-S-S protein?
1 answer-
MAK151 is capable of detecting reduced thiols like GSH, Cysteine, etc. It can also be used to detect SH groups in proteins, provided that known SH groups of the protein standard, such as BSA (0.5-1 GH/molecule), are available.
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