F0397

Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

• Synonym(s): Anti-CK16-Fluorescein isothiocyanate, Anti-FNEPPK, Anti-K16, Anti-K1CP, Anti-KRT16A, Anti-NEPPK, Anti-PC1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: PCK-26, monoclonal
• Application: IF (d), IHC (f)
• Citations: 16

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PCK-26, monoclonal
• form: buffered aqueous solution
• technique(s): direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (frozen sections): suitable
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.

FITC Monoclonal Anti-Pan Cytokeratin is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal anti-pan cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II, neutral-to-basic subfamily. Cytokeratin peptide 5 is the primary type II keratin in stratified epithelia while cytokeratin type 8 is a major type II keratin in simple epithelia. Cytokeratin 6 is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

Immunogen

cytokeratin from human epidermis.

Application

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse has been used in:

• immunohistochemistry
• staining chicken intestinal cells
• immunofluorescence staining

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse is suitable for direct immunofluorescence at a dilution of 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues and immunohistochemistry (frozen sections).

Biochem/physiol Actions

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)