E6412
Cellobiohydrolase I from Hypocrea jecorina
100 U/mg, recombinant, expressed in corn
Sustainability features
Synonym(s):
Cel7A, Cellobiosidase, Cellulase
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recombinant
expressed in corn
Quality Segment
form
liquid
specific activity
100 U/mg
sustainability
Sustainability features
shipped in
dry ice
storage temp.
−20°C
General description
Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.
This product aligns with one or more of the 12 Principles of Green Chemistry Find details here.
Application
Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.
Biochem/physiol Actions
Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles. Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.
Physical form
Provided as an ammonium sulfate precipitate with the source as recombinant maize.
Other Notes
Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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