B9879

BES

≥99.0% (titration)

• Synonym(s): 2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid, n,n-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
• Empirical Formula (Hill Notation): C₆H₁₅NO₅S
• CAS Number: 10191-18-1
• Molecular Weight: 213.25
• Beilstein: 1781572
• EC Number: 233-465-5
• MDL number: MFCD00007533
• UNSPSC Code: 12161700
• PubChem Substance ID: 24892162
• NACRES: NA.25

Properties

• Assay: ≥99.0% (titration)
• form: crystalline
• storage condition: dry at room temperature
• color: white
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• useful pH range: 6.4-7.8
• pKa (25 °C): 7.1
• solubility: water: 0.5 g/mL, clear, colorless
• density: 1.30 g/cm³ at 20—25 °C; 1.35 g/cm³ at 20—25 °C
• application(s): clinical research; diagnostic assay manufacturing; general analytical; life science and biopharma
• storage temp.: room temp
• SMILES string: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChI key: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Description

General description

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, known as BES, is a zwitterionic buffer and a valuable secondary standard in biochemical research. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. The useful pH range for BES buffer is 6.4 to 7.8. It is one of the "Good" buffers developed in the 1960s to provide buffering capacity in the pH range of 6.15 to 8.35, making it widely applicable in biochemical studies. BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.

Application

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Further, BES has found application in transfection studies, both in the preparation of reagents for transfection and in signaling assays related to Neural Melanocortin Receptors.

Features and Benefits

• Suitable for Cell Culture media and as a Buffering solution in Biochemical and Molecular Biology Research
• Effective Buffering from pH 2.5-5.0 (25 °C, 213.3 g/L) with a pKa of 7.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves