MABN1101
Anti-SRY, clone 1G4 Antibody
clone 1G4, from mouse
Synonym(s):
Sex-determining region Y protein, Testis-determining factor, SRY
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G4, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SRY(6736)
General description
Sex-determining region Y protein, also known as Testis-determining factor, and encoded by the human gene SRY or TDF is a transcriptional regulator that controls a genetic switch in male development. SRY is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) to Sertoli rather than granulosa cells. In male adult brain SRY is involved in the maintenance of motor functions of dopaminergic neurons. SRY is also involved in different aspects of gene regulation including promoter activation or repression, DNA bending and manipulation. The SRY HMG box recognizes DNA by partial intercalation into the minor groove, and SRY is also involved in pre-mRNA splicing. SRY is expressed in the developing testis and surrounding tissue and can be found expressed in neurons in the adult. SRY is nuclear associated.
Immunogen
Purified recombinant fragment of human SRY expressed in E. Coli
Application
Anti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SRY in cervical and ovarian cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in NTERA-2 cell lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.
Target description
~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Eugene Lee et al.
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Poor survival of grafted cells is the major impediment of successful cell-based therapies for bone regeneration. Implanted cells undergo rapid death in an ischemic environment largely because of hypoxia and metabolic stress from glucose deficiency. Understanding the intracellular metabolic processes
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