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Key Documents

MABC44

Sigma-Aldrich

Anti-LAMP-3 Antibody, clone 16H11.2

clone 16H11.2, from mouse

Synonym(s):

LAMP, CD208, LAMP-3, DC-loysosome-associated membrane glycoprotein, DC LAMP, DC-LAMP, Protein TSC403

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

16H11.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAMP3(27074)

General description

Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.

Immunogen

Recombinant protein corresponding to human LAMP-3.

Application

Detect LAMP-3 using this Anti-LAMP-3 Antibody, clone 16H11.2 validated for use in WB, IH.
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected LAMP-3 in colorectal adenocarcinoma tissue.
Research Category
Cell Structure
Research Sub Category
Apoptosis - Additional

Organelle & Cell Markers

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-3 in MCF-7 cell lysate.

Target description

~65 kDa observed.
The calculated molecular weight of this protein is 41 kDa, but has been observed at ~65-90 kDa due to glycosolation (de Saint-Vis, B., et al. (1998). Immunity. 9(3):325-336).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junya Nishimura et al.
Esophagus : official journal of the Japan Esophageal Society, 16(4), 333-344 (2019-04-11)
Dendritic cells (DCs) are the most potent antigen-presenting cells to induce cytotoxic T lymphocytes in the tumor environment. After acquiring antigens, DCs undergo maturation and their expression of MHC and co-stimulation molecules are enhanced, along with lysosome-associated membrane glycoprotein 3
Jorge Antolio Dominguez-Bautista et al.
European journal of cell biology, 94(3-4), 148-161 (2015-02-15)
The family of lysosome-associated membrane proteins (LAMP) includes the ubiquitously expressed LAMP1 and LAMP2, which account for half of the proteins in the lysosomal membrane. Another member of the LAMP family is LAMP3, which is expressed only in certain cell

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