05-389

Anti-Rac1 Antibody, clone 23A8

clone 23A8, Upstate^®, from mouse

• Synonym(s): Anti-Rac1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 23A8, monoclonal
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NM_006908
• UniProt accession no.: P63000
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... RAC1(5879)

Description

General description

Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.

Specificity

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

Immunogen

Recombinant protein containing the full length human Rac

Application

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

G-proteins

This Anti-Rac1 Antibody, clone 23A8 has been published in more than 40 citations and validated for use in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Rac1 protein.

Quality

routinely evaluated by immunoblot on rat brain microsomal protein preparation

Target description

21 kDa

Physical form

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1