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G005685

HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethyl acetate; [C] isopropanol; (85:10:5, A:B:C)
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 450 nm
injection: 10 μL
sample: 50-100 μg/mL hexane

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Ascentis silica is used in the analysis of naturally occurring carotenoid pigments in the form of alcohols, xanthophyll and astaxanthin.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Astaxanthin
    Xanthophyll

analytical column

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mobile phase component

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standard

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