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Ascentis® Express 90 Å C18 (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

surface area

90 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis® Express 5 μm C18 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the highly uniform overall particle size of 5-microns. The densely bonded, extensively endcapped dimethyloctadecyl stationary phase of Ascentis Express 5 μm C18 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Application

HPLC Analysis of Amine Medications on Ascentis® Express C18, 5 μm

HPLC Analysis of Pyrethrins on Ascentis® Express C18, 5 μm

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USBS002340
USBS002339
USBS002338
USBS002337
USBS002336

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Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Virginia Brighenti et al.
Journal of pharmaceutical and biomedical analysis, 143, 228-236 (2017-06-14)
The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)
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