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TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Synonym(s):

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

form

liquid

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

565 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

room temp

General description

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Application

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Biochem/physiol Actions

Dye binds to cellular protein and is then solubilized in base.

Signal Word

Danger

Hazard Statements

H226,H314,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

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Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Immune expression and inhibition of heat shock protein 90 in uveal melanoma
Faingold D, et al.
Clinical Cancer Research, 14(3), 847-855 (2008)
Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets
Miranda G, et al.
Neuro-Oncology, 15(2), 172-188 (2012)
Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
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