T9702

Anti-p8/TTD-A (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa (tagged fused protein)
• species reactivity: mouse, rat, human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human p8/TTD-A; western blot: 1-2 μg/mL using HEK-293T over-expressing human p8/TTD-A
• UniProt accession no.: Q6ZYL4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GTF2H5(404672) ; mouse ... Gtf2H5(66467) ; rat ... Gtf2h5(502227)

Description

General description

8-kDa trichothiodystrophy group A (p8/TTD-A) protein is the tenth subunit of general transcription factor IIH (TGFIIH). This protein exists as attached to transcription factor IIH (TFIIH) and as a free fraction that alternates between the cytoplasm and nucleus. p8/TTD-A gene is mapped on the human chromosome at 6q25.3.

Specificity

Anti-p8/TTD-A (C-terminal) specifically recognizes human, rat, and mouse p8/TTD-A.

Application

Anti-p8/TTD-A (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

8-kDa trichothiodystrophy group A (p8/TTD-A) protein defects leads to a sun-sensitive form of trichothiodystrophy (TTD), a rare autosomal recessive disorder. It is essential for the stability of the transcription factor IIH (TFIIH) complex. The cells from patients with p8/TTD-A have reduced levels of TFIIH while overexpressing p8/TTD-A restored the cellular level TFIIH complex.

Target description

p8/TTD-A (C-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves