SRP8009

GITRL, Soluble mouse

recombinant, expressed in HEK 293 cells, FLAG^® tagged, >90% (SDS-PAGE)

• Synonym(s): AITRL, EGF-like protein 9, Glucocorticoid-induced TNF receptor ligand, TNFSF18
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in HEK 293 cells
• tag: FLAG^® tagged
• Assay: >90% (SDS-PAGE)
• form: liquid
• mol wt: ~22 kDa by SDS-PAGE
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: ≥0.2 mg/mL
• impurities: <0.1 EU/μg endotoxin, tested
• color: clear
• UniProt accession no.: Q7TS55
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Tnfsf18(240873)

Description

General description

GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Physical form

Solution in 10 mM glycine, pH 10.0.

Other Notes

The extracellular domain of mouse GITRL (aa 45-173) is fused at the N-terminus to a FLAG^®-tag.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable