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recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
4.0-6.0 nmol/min·mg (radiometric)
mol wt
~70 kDa
concentration
0.1 μg/μL
accession no.
BC132833
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MYLK4(340156)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human MYLK4 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MYLK4 or myosin light chain kinase family, member 4 is Serine/Threonine protein kinases in which MYLK4 is one of four myosin light chain kinases in a comprehensive analysis of protein kinases encoded by the human genome.
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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