SAB3501195

Anti-TRIM5 alpha antibody produced in rabbit

affinity isolated antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: Q0PF16
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rhesus macaque ... TRIM5(574288)

Description

General description

TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.

Immunogen

TRIM5 alpha antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha.The immunogen is located within amino acids 390 - 440 of TRIM5 alpha.

Application

TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Physical form

Supplied in PBS containing 0.02% sodium azide.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable