S9319

Anti-α/β-SNAP (140-157) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD07370251
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, rat, mouse
• technique(s): western blot: 1:1,000-1:2,000 using cytosolic fraction S1 of rat and mouse brain
• UniProt accession no.: P54920; Q9H115
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NAPA(8775) , NAPB(63908) ; mouse ... Napa(108124) ; rat ... Napa(140673)

Description

General description

α-SNAP is 80% homologous to β-SNAP and is expressed in all mammalian tissues, whereas β-SNAP is expressed only in brain.

αSNAP (α-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) is widely present and abundantly expressed in all tissues. It is located on human chromosome 19q13.32. βSNAP (β-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) is expressed only in neuronal tissues and is located on human chromosome 20p11.21.

Specificity

Anti-α/β-SNAP (KS-18) recognizes α/β-SNAP.

Immunogen

synthetic peptide encoding amino acids 140-157 located near the mid-region of mouse α-SNAP, conjugated to KLH. The sequence is identical in human, rat, and bovine α-SNAP and in human, mouse, rat and bovine β-SNAP.

Application

Anti-α/β-SNAP (140-157) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

α-SNAP binding to the soluble NSF attachment protein receptor (SNARE) complex, recruits NSF to form a 20S complex. ATP hydrolysis by NSF ATPase activity induces disassembly of the 20S complex, thus facilitating vesicle fusion to the membrane and exocytosis.

α-soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein (α-SNAP) is an essential constituent of the membrane fusion machinery. It plays an important role in insulin exocytosis through large dense core vesicles. In human epithelia, absence of αSNAP promotes non-canonical autophagy. α-SNAP and NSF are highly essential in the initial stage of human sperm acrosome reaction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable